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Journal: The Journal of Biological Chemistry
Article Title: ARF6 controls VSMC phenotypic switching upon lipid stimulation to promote inflammatory signaling contributing to the progression of atherosclerosis
doi: 10.1016/j.jbc.2026.111165
Figure Lengend Snippet: ARF6 knockout affects local inflammation in the plaque . A , images of aortic arch cross-sections stained with H&E. Arrows identify the foam cells. n = 5 animals per group. The scale bar represents 100 μm. B , total RNA was extracted from isolated mouse abdominal aortic VSMCs with the Qiagen RNeasy kit according to the manufacturer’s instructions. Quantifications are the mean ± SD of six or seven animals per group (three animals per group for Il1β ). ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05, ns, not significant (unpaired Student’s t test). C , representative aortic sinus cross-sections stained for immunohistochemistry with anti-CD45. n = 5 animals per group. The scale bar represents 250 μm. D , immunofluorescence of frozen aortic sinus stained with anti-CD68 and Alexa Fluor 568. Nuclei were stained with DAPI. Images are representative of three nonconstitutive sections per animal, n = 5 per group. Quantifications are the mean ± SD of six or eight lesions per group. ns, not significant (unpaired Student’s t test). E–F , immunofluorescence of frozen aortic sinus stained with anti-ICAM-1 ( E ) or anti-VCAM-1 ( F ) and Alexa Fluor 568. Nuclei were stained with DAPI. Images are representative of three nonconstitutive sections per animal, n = 5 per group. Quantifications are the mean ± SD of 8 to 11 lesions per group. ∗ p < 0.05 (unpaired Student’s t test). Dotted lines encircle the lesions. The scale bar represents 100 μm. ARF, ADP-ribosylation factorl; ICAM, intracellular adhesion molecule; VCAM, vascular cell adhesion molecule; VSMC, vascular smooth muscle cell.
Article Snippet:
Techniques: Knock-Out, Staining, Isolation, Immunohistochemistry, Immunofluorescence